Which gene on the pARA-R plasmid codes for red fluorescent protein?

Which gene on the pARA-R plasmid codes for red fluorescent protein?

• A. rfp
• B. pbad
• C. araC
• D. ampr

Answer: (A)

Red fluorescent protein is produced by the gene called rfp. On pARA-R, the rfp gene sits under the control of the arabinose-inducible pBAD promoter and is regulated by the AraC protein. When arabinose is present, AraC activates transcription from pBAD, leading to expression of RFP and visible red fluorescence. The other items are not the fluorescent protein gene: pBAD is the promoter, araC is the regulatory protein, and ampr provides antibiotic resistance for selection.

Red fluorescent protein is produced by the gene called rfp. On pARA-R, the rfp gene sits under the control of the arabinose-inducible pBAD promoter and is regulated by the AraC protein. When arabinose is present, AraC activates transcription from pBAD, leading to expression of RFP and visible red fluorescence. The other items are not the fluorescent protein gene: pBAD is the promoter, araC is the regulatory protein, and ampr provides antibiotic resistance for selection.