What are the sticky ends generated by BamHI and HindIII?

What are the sticky ends generated by BamHI and HindIII?

• A. BamHI produces a AGCT overhang; HindIII produces a GATC overhang
• B. BamHI produces a GATC overhang; HindIII produces an AGCT overhang
• C. BamHI produces a TCGA overhang; HindIII produces a CTAG overhang
• D. BamHI produces a CCGG overhang; HindIII produces a AATT overhang

Answer: (B)

Sticky ends come from staggered cuts by restriction enzymes, leaving short single-stranded overhangs that can base-pair with complementary sequences. BamHI recognizes GGATCC and cuts between the first two Gs (G/GATCC), producing a 4-base 5' overhang of GATC. HindIII recognizes AAGCTT and cuts between the two As (A/A GCTT), producing a 4-base 5' overhang of AGCT. So BamHI yields GATC as its overhang and HindIII yields AGCT.

Sticky ends come from staggered cuts by restriction enzymes, leaving short single-stranded overhangs that can base-pair with complementary sequences. BamHI recognizes GGATCC and cuts between the first two Gs (G/GATCC), producing a 4-base 5' overhang of GATC. HindIII recognizes AAGCTT and cuts between the two As (A/A GCTT), producing a 4-base 5' overhang of AGCT. So BamHI yields GATC as its overhang and HindIII yields AGCT.